focused ion beam scanning electron microscopy (fib-sem) helios nanolab 650 Search Results


focused ion beam scanning electron microscopy helios nanolab 600i  (NanoLab Inc)
90
NanoLab Inc focused ion beam scanning electron microscopy helios nanolab 600i
Volumetric reconstruction of projection neuron boutons in a reference region of the mushroom body calyx (A) Schematic of olfactory projection neurons (PNs) in Drosophila . PNs connect the antenna lobe (AL) to the mushroom body (MB) and lateral horn (LH). The calyx is the input region of the MB. (B) Posterior view of the calyx under Focused Ion Beam Scanning Electron <t>Microscopy</t> (FIB-SEM). PN processes and the soma of MB Kenyon cell (KC) are circled by green dotted lines, and the calyx of the MB is circled by yellow dotted lines. Scale bar = 10 μm. (C) Raw images from FIB-SEM in the center of the calyx in a volume of 20 × 20 × 25 μm. (D) Workflow of volumetric reconstruction of PN axonal boutons. A PN bouton was identified on individual sections and the centers of consecutive sections were connected to generate its skeleton (D1). Sections of one PN bouton were filled with the identical color to depict its shape (D2). Presynaptic active zones (PAZs, blue dots) and dense core vesicles (DCVs, green dots) were annotated in every bouton (D1 and D3). Scale bars = 5 μm. (E) Overall view of EM-reconstructed PN boutons. 89 reconstructed PN boutons were filled with different colors. Scale bars = 5 μm.
Focused Ion Beam Scanning Electron Microscopy Helios Nanolab 600i, supplied by NanoLab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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focused ion beam–electron beam double beam system  (NanoLab Inc)
90
NanoLab Inc focused ion beam–electron beam double beam system
Volumetric reconstruction of projection neuron boutons in a reference region of the mushroom body calyx (A) Schematic of olfactory projection neurons (PNs) in Drosophila . PNs connect the antenna lobe (AL) to the mushroom body (MB) and lateral horn (LH). The calyx is the input region of the MB. (B) Posterior view of the calyx under Focused Ion Beam Scanning Electron <t>Microscopy</t> (FIB-SEM). PN processes and the soma of MB Kenyon cell (KC) are circled by green dotted lines, and the calyx of the MB is circled by yellow dotted lines. Scale bar = 10 μm. (C) Raw images from FIB-SEM in the center of the calyx in a volume of 20 × 20 × 25 μm. (D) Workflow of volumetric reconstruction of PN axonal boutons. A PN bouton was identified on individual sections and the centers of consecutive sections were connected to generate its skeleton (D1). Sections of one PN bouton were filled with the identical color to depict its shape (D2). Presynaptic active zones (PAZs, blue dots) and dense core vesicles (DCVs, green dots) were annotated in every bouton (D1 and D3). Scale bars = 5 μm. (E) Overall view of EM-reconstructed PN boutons. 89 reconstructed PN boutons were filled with different colors. Scale bars = 5 μm.
Focused Ion Beam–Electron Beam Double Beam System, supplied by NanoLab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fib/sem with an electron beam voltage of 5 kv  (NanoLab Inc)
90
NanoLab Inc fib/sem with an electron beam voltage of 5 kv
Volumetric reconstruction of projection neuron boutons in a reference region of the mushroom body calyx (A) Schematic of olfactory projection neurons (PNs) in Drosophila . PNs connect the antenna lobe (AL) to the mushroom body (MB) and lateral horn (LH). The calyx is the input region of the MB. (B) Posterior view of the calyx under Focused Ion Beam Scanning Electron <t>Microscopy</t> (FIB-SEM). PN processes and the soma of MB Kenyon cell (KC) are circled by green dotted lines, and the calyx of the MB is circled by yellow dotted lines. Scale bar = 10 μm. (C) Raw images from FIB-SEM in the center of the calyx in a volume of 20 × 20 × 25 μm. (D) Workflow of volumetric reconstruction of PN axonal boutons. A PN bouton was identified on individual sections and the centers of consecutive sections were connected to generate its skeleton (D1). Sections of one PN bouton were filled with the identical color to depict its shape (D2). Presynaptic active zones (PAZs, blue dots) and dense core vesicles (DCVs, green dots) were annotated in every bouton (D1 and D3). Scale bars = 5 μm. (E) Overall view of EM-reconstructed PN boutons. 89 reconstructed PN boutons were filled with different colors. Scale bars = 5 μm.
Fib/Sem With An Electron Beam Voltage Of 5 Kv, supplied by NanoLab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dual-beam fib-sem fo- used ion beam scanning electron microscope  (NanoLab Inc)
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NanoLab Inc dual-beam fib-sem fo- used ion beam scanning electron microscope
Volumetric reconstruction of projection neuron boutons in a reference region of the mushroom body calyx (A) Schematic of olfactory projection neurons (PNs) in Drosophila . PNs connect the antenna lobe (AL) to the mushroom body (MB) and lateral horn (LH). The calyx is the input region of the MB. (B) Posterior view of the calyx under Focused Ion Beam Scanning Electron <t>Microscopy</t> (FIB-SEM). PN processes and the soma of MB Kenyon cell (KC) are circled by green dotted lines, and the calyx of the MB is circled by yellow dotted lines. Scale bar = 10 μm. (C) Raw images from FIB-SEM in the center of the calyx in a volume of 20 × 20 × 25 μm. (D) Workflow of volumetric reconstruction of PN axonal boutons. A PN bouton was identified on individual sections and the centers of consecutive sections were connected to generate its skeleton (D1). Sections of one PN bouton were filled with the identical color to depict its shape (D2). Presynaptic active zones (PAZs, blue dots) and dense core vesicles (DCVs, green dots) were annotated in every bouton (D1 and D3). Scale bars = 5 μm. (E) Overall view of EM-reconstructed PN boutons. 89 reconstructed PN boutons were filled with different colors. Scale bars = 5 μm.
Dual Beam Fib Sem Fo Used Ion Beam Scanning Electron Microscope, supplied by NanoLab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fib-sem instrument fei nova 200  (NanoLab Inc)
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Volumetric reconstruction of projection neuron boutons in a reference region of the mushroom body calyx (A) Schematic of olfactory projection neurons (PNs) in Drosophila . PNs connect the antenna lobe (AL) to the mushroom body (MB) and lateral horn (LH). The calyx is the input region of the MB. (B) Posterior view of the calyx under Focused Ion Beam Scanning Electron <t>Microscopy</t> (FIB-SEM). PN processes and the soma of MB Kenyon cell (KC) are circled by green dotted lines, and the calyx of the MB is circled by yellow dotted lines. Scale bar = 10 μm. (C) Raw images from FIB-SEM in the center of the calyx in a volume of 20 × 20 × 25 μm. (D) Workflow of volumetric reconstruction of PN axonal boutons. A PN bouton was identified on individual sections and the centers of consecutive sections were connected to generate its skeleton (D1). Sections of one PN bouton were filled with the identical color to depict its shape (D2). Presynaptic active zones (PAZs, blue dots) and dense core vesicles (DCVs, green dots) were annotated in every bouton (D1 and D3). Scale bars = 5 μm. (E) Overall view of EM-reconstructed PN boutons. 89 reconstructed PN boutons were filled with different colors. Scale bars = 5 μm.
Fib Sem Instrument Fei Nova 200, supplied by NanoLab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dual-beam fib/scanning electron microscope (sem) instrument  (NanoLab Inc)
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NanoLab Inc dual-beam fib/scanning electron microscope (sem) instrument
Volumetric reconstruction of projection neuron boutons in a reference region of the mushroom body calyx (A) Schematic of olfactory projection neurons (PNs) in Drosophila . PNs connect the antenna lobe (AL) to the mushroom body (MB) and lateral horn (LH). The calyx is the input region of the MB. (B) Posterior view of the calyx under Focused Ion Beam Scanning Electron <t>Microscopy</t> (FIB-SEM). PN processes and the soma of MB Kenyon cell (KC) are circled by green dotted lines, and the calyx of the MB is circled by yellow dotted lines. Scale bar = 10 μm. (C) Raw images from FIB-SEM in the center of the calyx in a volume of 20 × 20 × 25 μm. (D) Workflow of volumetric reconstruction of PN axonal boutons. A PN bouton was identified on individual sections and the centers of consecutive sections were connected to generate its skeleton (D1). Sections of one PN bouton were filled with the identical color to depict its shape (D2). Presynaptic active zones (PAZs, blue dots) and dense core vesicles (DCVs, green dots) were annotated in every bouton (D1 and D3). Scale bars = 5 μm. (E) Overall view of EM-reconstructed PN boutons. 89 reconstructed PN boutons were filled with different colors. Scale bars = 5 μm.
Dual Beam Fib/Scanning Electron Microscope (Sem) Instrument, supplied by NanoLab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dual-beam fib/scanning electron microscope (sem) instrument - by Bioz Stars, 2026-10
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jem 2100 field emission gun transmission electron microscope  (JEOL)
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JEOL jem 2100 field emission gun transmission electron microscope
Volumetric reconstruction of projection neuron boutons in a reference region of the mushroom body calyx (A) Schematic of olfactory projection neurons (PNs) in Drosophila . PNs connect the antenna lobe (AL) to the mushroom body (MB) and lateral horn (LH). The calyx is the input region of the MB. (B) Posterior view of the calyx under Focused Ion Beam Scanning Electron <t>Microscopy</t> (FIB-SEM). PN processes and the soma of MB Kenyon cell (KC) are circled by green dotted lines, and the calyx of the MB is circled by yellow dotted lines. Scale bar = 10 μm. (C) Raw images from FIB-SEM in the center of the calyx in a volume of 20 × 20 × 25 μm. (D) Workflow of volumetric reconstruction of PN axonal boutons. A PN bouton was identified on individual sections and the centers of consecutive sections were connected to generate its skeleton (D1). Sections of one PN bouton were filled with the identical color to depict its shape (D2). Presynaptic active zones (PAZs, blue dots) and dense core vesicles (DCVs, green dots) were annotated in every bouton (D1 and D3). Scale bars = 5 μm. (E) Overall view of EM-reconstructed PN boutons. 89 reconstructed PN boutons were filled with different colors. Scale bars = 5 μm.
Jem 2100 Field Emission Gun Transmission Electron Microscope, supplied by JEOL, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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jem 2100 field emission gun transmission electron microscope - by Bioz Stars, 2026-10
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fei talos nanolab g3 uc fib-sem  (NanoLab Inc)
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NanoLab Inc fei talos nanolab g3 uc fib-sem
Volumetric reconstruction of projection neuron boutons in a reference region of the mushroom body calyx (A) Schematic of olfactory projection neurons (PNs) in Drosophila . PNs connect the antenna lobe (AL) to the mushroom body (MB) and lateral horn (LH). The calyx is the input region of the MB. (B) Posterior view of the calyx under Focused Ion Beam Scanning Electron <t>Microscopy</t> (FIB-SEM). PN processes and the soma of MB Kenyon cell (KC) are circled by green dotted lines, and the calyx of the MB is circled by yellow dotted lines. Scale bar = 10 μm. (C) Raw images from FIB-SEM in the center of the calyx in a volume of 20 × 20 × 25 μm. (D) Workflow of volumetric reconstruction of PN axonal boutons. A PN bouton was identified on individual sections and the centers of consecutive sections were connected to generate its skeleton (D1). Sections of one PN bouton were filled with the identical color to depict its shape (D2). Presynaptic active zones (PAZs, blue dots) and dense core vesicles (DCVs, green dots) were annotated in every bouton (D1 and D3). Scale bars = 5 μm. (E) Overall view of EM-reconstructed PN boutons. 89 reconstructed PN boutons were filled with different colors. Scale bars = 5 μm.
Fei Talos Nanolab G3 Uc Fib Sem, supplied by NanoLab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fib-sem  (NanoLab Inc)
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NanoLab Inc fib-sem
Volumetric reconstruction of projection neuron boutons in a reference region of the mushroom body calyx (A) Schematic of olfactory projection neurons (PNs) in Drosophila . PNs connect the antenna lobe (AL) to the mushroom body (MB) and lateral horn (LH). The calyx is the input region of the MB. (B) Posterior view of the calyx under Focused Ion Beam Scanning Electron <t>Microscopy</t> (FIB-SEM). PN processes and the soma of MB Kenyon cell (KC) are circled by green dotted lines, and the calyx of the MB is circled by yellow dotted lines. Scale bar = 10 μm. (C) Raw images from FIB-SEM in the center of the calyx in a volume of 20 × 20 × 25 μm. (D) Workflow of volumetric reconstruction of PN axonal boutons. A PN bouton was identified on individual sections and the centers of consecutive sections were connected to generate its skeleton (D1). Sections of one PN bouton were filled with the identical color to depict its shape (D2). Presynaptic active zones (PAZs, blue dots) and dense core vesicles (DCVs, green dots) were annotated in every bouton (D1 and D3). Scale bars = 5 μm. (E) Overall view of EM-reconstructed PN boutons. 89 reconstructed PN boutons were filled with different colors. Scale bars = 5 μm.
Fib Sem, supplied by NanoLab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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focused ion beam milling-scanning electron microscopy  (NanoLab Inc)
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NanoLab Inc focused ion beam milling-scanning electron microscopy
Volumetric reconstruction of projection neuron boutons in a reference region of the mushroom body calyx (A) Schematic of olfactory projection neurons (PNs) in Drosophila . PNs connect the antenna lobe (AL) to the mushroom body (MB) and lateral horn (LH). The calyx is the input region of the MB. (B) Posterior view of the calyx under Focused Ion Beam Scanning Electron <t>Microscopy</t> (FIB-SEM). PN processes and the soma of MB Kenyon cell (KC) are circled by green dotted lines, and the calyx of the MB is circled by yellow dotted lines. Scale bar = 10 μm. (C) Raw images from FIB-SEM in the center of the calyx in a volume of 20 × 20 × 25 μm. (D) Workflow of volumetric reconstruction of PN axonal boutons. A PN bouton was identified on individual sections and the centers of consecutive sections were connected to generate its skeleton (D1). Sections of one PN bouton were filled with the identical color to depict its shape (D2). Presynaptic active zones (PAZs, blue dots) and dense core vesicles (DCVs, green dots) were annotated in every bouton (D1 and D3). Scale bars = 5 μm. (E) Overall view of EM-reconstructed PN boutons. 89 reconstructed PN boutons were filled with different colors. Scale bars = 5 μm.
Focused Ion Beam Milling Scanning Electron Microscopy, supplied by NanoLab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scanning electron microscope sem  (NanoLab Inc)
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NanoLab Inc scanning electron microscope sem
Volumetric reconstruction of projection neuron boutons in a reference region of the mushroom body calyx (A) Schematic of olfactory projection neurons (PNs) in Drosophila . PNs connect the antenna lobe (AL) to the mushroom body (MB) and lateral horn (LH). The calyx is the input region of the MB. (B) Posterior view of the calyx under Focused Ion Beam Scanning Electron <t>Microscopy</t> (FIB-SEM). PN processes and the soma of MB Kenyon cell (KC) are circled by green dotted lines, and the calyx of the MB is circled by yellow dotted lines. Scale bar = 10 μm. (C) Raw images from FIB-SEM in the center of the calyx in a volume of 20 × 20 × 25 μm. (D) Workflow of volumetric reconstruction of PN axonal boutons. A PN bouton was identified on individual sections and the centers of consecutive sections were connected to generate its skeleton (D1). Sections of one PN bouton were filled with the identical color to depict its shape (D2). Presynaptic active zones (PAZs, blue dots) and dense core vesicles (DCVs, green dots) were annotated in every bouton (D1 and D3). Scale bars = 5 μm. (E) Overall view of EM-reconstructed PN boutons. 89 reconstructed PN boutons were filled with different colors. Scale bars = 5 μm.
Scanning Electron Microscope Sem, supplied by NanoLab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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scanning electron microscopic images fei nova nanolab 600 fib/sem  (NanoLab Inc)
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NanoLab Inc scanning electron microscopic images fei nova nanolab 600 fib/sem
( a ) Particle size and zeta potential analysis of the native and FITC-p11 peptide-encapsulated nanoparticles. ( b ) (i) SEM (scanning electron microscopy) image of nanoparticle. (ii) High magnification SEM image. (iii) Confocal <t>microscopic</t> image of the FITC-P11 peptide-encapsulated nanoparticles.
Scanning Electron Microscopic Images Fei Nova Nanolab 600 Fib/Sem, supplied by NanoLab Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Volumetric reconstruction of projection neuron boutons in a reference region of the mushroom body calyx (A) Schematic of olfactory projection neurons (PNs) in Drosophila . PNs connect the antenna lobe (AL) to the mushroom body (MB) and lateral horn (LH). The calyx is the input region of the MB. (B) Posterior view of the calyx under Focused Ion Beam Scanning Electron Microscopy (FIB-SEM). PN processes and the soma of MB Kenyon cell (KC) are circled by green dotted lines, and the calyx of the MB is circled by yellow dotted lines. Scale bar = 10 μm. (C) Raw images from FIB-SEM in the center of the calyx in a volume of 20 × 20 × 25 μm. (D) Workflow of volumetric reconstruction of PN axonal boutons. A PN bouton was identified on individual sections and the centers of consecutive sections were connected to generate its skeleton (D1). Sections of one PN bouton were filled with the identical color to depict its shape (D2). Presynaptic active zones (PAZs, blue dots) and dense core vesicles (DCVs, green dots) were annotated in every bouton (D1 and D3). Scale bars = 5 μm. (E) Overall view of EM-reconstructed PN boutons. 89 reconstructed PN boutons were filled with different colors. Scale bars = 5 μm.

Journal: iScience

Article Title: Classifying Drosophila olfactory projection neuron boutons by quantitative analysis of electron microscopic reconstruction

doi: 10.1016/j.isci.2022.104180

Figure Lengend Snippet: Volumetric reconstruction of projection neuron boutons in a reference region of the mushroom body calyx (A) Schematic of olfactory projection neurons (PNs) in Drosophila . PNs connect the antenna lobe (AL) to the mushroom body (MB) and lateral horn (LH). The calyx is the input region of the MB. (B) Posterior view of the calyx under Focused Ion Beam Scanning Electron Microscopy (FIB-SEM). PN processes and the soma of MB Kenyon cell (KC) are circled by green dotted lines, and the calyx of the MB is circled by yellow dotted lines. Scale bar = 10 μm. (C) Raw images from FIB-SEM in the center of the calyx in a volume of 20 × 20 × 25 μm. (D) Workflow of volumetric reconstruction of PN axonal boutons. A PN bouton was identified on individual sections and the centers of consecutive sections were connected to generate its skeleton (D1). Sections of one PN bouton were filled with the identical color to depict its shape (D2). Presynaptic active zones (PAZs, blue dots) and dense core vesicles (DCVs, green dots) were annotated in every bouton (D1 and D3). Scale bars = 5 μm. (E) Overall view of EM-reconstructed PN boutons. 89 reconstructed PN boutons were filled with different colors. Scale bars = 5 μm.

Article Snippet: Serial EM images were then acquired by focused ion beam scanning electron microscopy (FIB-SEM, Helios Nanolab 600i), which is controlled by Auto Slice & View software.

Techniques: Electron Microscopy

( a ) Particle size and zeta potential analysis of the native and FITC-p11 peptide-encapsulated nanoparticles. ( b ) (i) SEM (scanning electron microscopy) image of nanoparticle. (ii) High magnification SEM image. (iii) Confocal microscopic image of the FITC-P11 peptide-encapsulated nanoparticles.

Journal: Pharmaceutics

Article Title: An Injectable Nano-Enabled Thermogel to Attain Controlled Delivery of p11 Peptide for the Potential Treatment of Ocular Angiogenic Disorders of the Posterior Segment

doi: 10.3390/pharmaceutics13020176

Figure Lengend Snippet: ( a ) Particle size and zeta potential analysis of the native and FITC-p11 peptide-encapsulated nanoparticles. ( b ) (i) SEM (scanning electron microscopy) image of nanoparticle. (ii) High magnification SEM image. (iii) Confocal microscopic image of the FITC-P11 peptide-encapsulated nanoparticles.

Article Snippet: Scanning electron microscopic images (FEI Nova Nanolab 600 FIB/SEM, Hillsboro, OR, USA) were acquired for the analysis of the morphology of the nanoparticles.

Techniques: Zeta Potential Analyzer, Electron Microscopy